TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristicsSource or reference
Strains
    DH5αλ φ80δ/lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK+) supE44 thi-1 gyrA relA1Gibco-BRL
    A136Strain C58 cured of pTiC5822
    A348A136 containing octopine-type Ti plasmid pTiA6NC22
    A348SpcrA348 mutated to Spcr39
    PC1000A348 derivative with the virB operon from Ti plasmid pTiA6NC deleted22
    Mx355A348 with a virD4 null mutation46
    At12516A348 with a virE2 null mutation24
    LBA4404T-DNA deletion strain36
    PC2000A348 with a tatC null mutationThis study
Plasmids
    pBSK+.NdeICrbr, pBSIISK+ containing an NdeI site at the translational start site of lacZ5
    pCR2.1-TOPOCrbr Kanr, pBR322 replicon, TA cloning vectorInvitrogen
    pPC914KSCrbr, pBKS+.NdeI with PvirB-B15
    pSW172Tetr, IncP derivative with pIC polylinker14
    pWM1487Crbr, pBR322 replicon with PBAD-torA::GFPW. Margolin
    pMMB22Crbr, IncQ plasmid broad-host-range cloning vector3
    pML122GenrGenr, RSF1010 derivative with a Genr cassette24
    pUC4KCrbr Kanr, pUC plasmid with a Kanr gene cassettePharmacia
    pBB50Cmr Kanr, pBCSK+ with Kanr and sacB genes5
    pZD29Crbr Kanr, pCR2.1-TOPO with tatC geneThis study
    pZD26Crbr, pBSK+.NdeI with tatCThis study
    pZD36Crbr, pBSK+.NdeI with Plac-tatCThis study
    pZD44Crbr, pBSK+.NdeI with PvirB-tatCThis study
    pZD51Crbr, pMMB22 with Ptac-torA::GFPThis study
    pZD31Crbr Kanr, pBR322 replicon carrying tatC with a Kanr insertionThis study
    pZD41Crbr Kanr, pBR322 replicon carrying sacB and tatC with a Kanr insertionThis study