TABLE 3.

Holdfast FITC-WGA labeling and rosette formation of holdfast biogenesis mutants

Strain (mutant gene)% of predivisional cells with FITC-WGA bindingaPresence of:
Holdfast sheddingbRosette formation
CB1578+
NA10000
YB2861 (pleC100)1
YB2862 (hfsB101)0
YB2863 (podJ102)7
YB2864 (podJ103)1
YB2865 (hfsA106)2
YB2866 (podJ107)2
YB2867 (podJ108)0
YB2868 (pleC109)0
YB2869 (hfsA111)0
YB2870 (hfsA118)0
YB2877 (hfsB176)3
YB2878 (hfsA125)NDcNDND
YB2879 (hfsD172)33+
YB2779 (hfaB113)2+
YB2779 (hfaB113)/plac290hfa7ND ND+
YB2780 (hfaB115)3+
YB2780 (hfaB115)/plac290hfa7ND ND+
YB2781 (hfa-120)15++
YB2781 (hfa-120)/plac290hfa7ND ND+
YB2782 (hfa-121)2+
YB2782 (hfa-121)/plac290hfa7ND ND+
YB2783 (hfa-122)0+
YB2783 (hfa-122)/plac290hfa7ND ND+
YB2784 (hfaB123)2+
YB2784 (hfaB123)/plac290hfa7ND ND+
YB3738 (hfa-105)31++
YB3738 (hfa-105)/plac290hfa7ND ND+
  • a FITC-WGA-labeled predivisional cells were counted for every 100 predivisional cells. Lectin binding experiments were performed three times.

  • b The number of shed holdfasts for every strain was compared to the number of shed holdfasts per count of 100 predivisional cells for the holdfast-negative strain NA1000.

  • c ND, not determined.