TABLE 1.

Bacterial strains and plasmids

Strains or plasmidGenotypeReference or source
V. cholerae
    N16961SmEl Tor strain isolated from the 7th pandemicLaboratory collection
    KFV11ΔmshA1 25
    ZK2736 flgF::Tn10Km 26
    ZK2987ΔmbaAThis study
    ZK2988ΔmbaA ΔmshA1This study
    ZK2989ΔmbaA flgF::Tn10KanThis study
    ZK2985 Plac::gfp::lacZJ. Zhu and J.J. Mekalanos
    ZK2979ΔmshA1 Plac::gfp::lacZThis study
    ZK2982 flgF::Tn10Kan Plac::gfp::lacZThis study
    ZK2984ΔmbaA Plac::gfp::lacZThis study
    ZK2990ΔmbaA ΔmshA1 Plac::gfp::lacZThis study
    ZK2991ΔmbaA flgF::Tn10Kan Plac::gfp::lacZThis study
    ZK3046 vps59::pGp704This study
    ZK3047 vpsR::pGp704This study
    ZK3048ΔmbaA vps59::pGp704This study
    ZK3049ΔmbaA vpsR::pGp704This study
E. coli
    DH5α λpir endA1 hdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF) U169 (φ80lacZΔM15) λpirR6KW. Lin and J.J. Mekalanos
    SM10 λpir thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpirR6K Kmr 15
Plasmids
    pBluescriptII KS+cloning vector; AprLaboratory collection
    pCVD442 oriR6K mobRP4 sacB Apr 8
    pJZ111 Plac::gfp::lacZ in pCVD442J. Zhu and J.J. Mekalanos
    pKEK349vps59′ in pGp704 28
    pNB1mbaA1′ in pBluescriptII KS+This study
    pNB3mbaA2′ in pBluescriptII KS+This study
    pNB4ΔmbaA in pWM91This study
    pvpsRvpsR′ in pGp704 31
    pWM91 oriR6K oriT sacB lacZα Apr 14