TABLE 3.

PAO1 sequence coordinates for regions of high sequence diversitya

Coordinates ofbLength (bp)cNo. of SNPsdNo. of bases availablee% SNPsfPAO1 ORFsg
PAO1 startPAO1 end
2900002949995,000831,4885.58PA0259-PA0262
3200003249995,000971,7545.53PA0285-PA0289
5150005199995,0001432,1036.80PA0457-PA0459
5300005349995,0001112,2884.85PA0470-PA0473
5550005599995,000661,2525.27PA0495-PA0500
6525006574995,000761,1716.49PA0594-PA0596
6850006899995,000447895.58PA0625-PA0633
6950006999995,000841,4365.85PA0640-PA0643
79000079999910,0002742,53510.81PA0719-PA0731
106000010649995,000579266.16PA0976-PA0982
117000011749995,0002241,88111.91PA1084-PA1087
215000021549995,0008884210.45PA1967-PA1972
255000025549995,000591,3464.38PA2312-PA2317
2635000265499920,0004515,7877.79PA2383-PA2397
266000026649995,00011555120.87PA2399
267250026774995,0001861,13216.43PA2402
2682500269249910,0001391,7238.07PA2402-PA2409
364750036524995,000811,4175.72PA3260-PA3264
406000040649995,000851,6955.01PA3624-PA3629
503750050424995,000981,9235.10PA4500-PA4503
507000050749995,0001622,0307.98PA4526-PA4532
509750051024995,0001191,8256.52PA4549-PA4554
518750051924995,000911,5615.83PA4625
572250057274995,0001621,9668.24PA5084-PA5089
609000060949995,0009361815.05PA5412-PA5415
  • a Sequencing data for all three strains was compared to the PAO1 reference in 5,000-bp sliding windows that were sequentially offset by 2,500 bp. Regions with at least 500 bp of alignable sequence that exhibited nucleotide diversity values greater than three standard deviations (>4.35% sequence differences) from the mean value of 0.5% are shown.

  • b Sequence coordinates of the PAO1 refence sequence, accessible at www.pseudomonas.com.

  • c Overall span of the PAO1 region encompassed by high sequence variation.

  • d Number of SNPs detected.

  • e Total number of alignable bases in which SNPs were detected.

  • f Percent SNPs among alignable bases.

  • g Annotated ORFs within high-diversity regions. A more comprehensive description of these genes is available at www.genome.washington.edu/UWGC and at www.pseudomonas.com.