TABLE 1.

Bacterial strains and plasmids

Strain or plasmidDescriptionSource or reference
Strains
    CodonPlusE. coli BL21-CodonPlus-(DE3)-RIL [F ompT hsdS(rB mB) dcm+ Tetr gal λ (DE3) endA Hte (argU ileY leuW Camr)]Stratagene
    ZK1746E. coli W3110 tna2 ΔlacU169 Δara ΔmurA::kan (pBAD30-Z)4
    ATM580CodonPlus transformed with pAJM5This work
    ATM581ATM580 ΔmurAEc::kanThis work
    ATM586CodonPlus transformed with pAJM6This work
    ATM587ATM586 ΔmurACt::kanThis work
    2457TS. flexneri 2a wild type8
    C. trachomatisC. trachomatis serovar L2H. Caldwell
Plasmids
    pBAD30Arabinose-inducible expression vector; Ampr; p15A origin; low copy number12
    pBAD33Arabinose-inducible expression vector; Cmr; p15A origin; low copy number12
    pBAD18Arabinose-inducible expression vector; Ampr; pMB1 origin; moderate copy number12
    pBAD30-ZpBAD30::murAEc; coding sequence and RBSa of murA from E. coli (W3110)4
    pAJM5pBAD18::murAEc; coding sequence and RBS of murA from E. coli (W3110)This work
    pAJM6pBAD18::murACt; coding sequence and RBS of murA from C. trachomatis serovar L2This work
  • a RBS, ribosomal binding site.