TABLE 2.

Effects of alanine substitutions on sporulation efficiency and localization of SpoVM-GFPa

PositionEffect on sporulationSpoVM-GFP localization
2Severe+
3Severe±
4Severe+
5None+
6Severe±
7None+
8Severe+
9Severe
10Mild+
11Intermediate+
12Severe+
13None+
14None+
15Severe+
16Intermediate+
17None+
19Mild+
20Intermediate+
21None+
22None+
23Mild+
24Mild+
25Intermediate+
26Mild+
  • a The sporulation efficiency of spoVM alanine substitution mutants was measured as described in Materials and Methods. The mutant strains contained a single copy of spoVM that was not fused to gfp. The efficiency of the wild type was set at 1.0. Severe, sporulation efficiency of <10−5 relative to the wild type; intermediate, efficiency of 10−2 to 10−5; mild, efficiency of 0.5 to 10−2; none, efficiency of >0.5. Subcellular localization was determined by fluorescence microscopy of strains producing fusions of SpoVM substitution mutants with GFP. Localization was scored on the basis of the extent to which fluorescence was restricted to the polar septum. +, pattern similar to that of the wild type; ±, localization to the cytoplasmic membrane detected; −, fusion protein more or less uniformly distributed along all membranes.