TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)aReference
A. actinomycetemcomitans strains
    CU1000Wild-type clinical isolate8
    CU1000NSpontaneous nalidixic acid-resistant mutant of CU100014
    JK1002CU1000N wzt::IS903φkan; Kmr19
    JK1017CU1000N rmlA::IS903φkan; KmrThis study
    JK1022CU1000N dspA::IS903φkan; Kmr19
Plasmids
    pJAK16Broad-host-range, mobilizable IncQ vector; Cmr; tac promoter, lacIq32
    pJK595pJAK16 with wzt of CU1000 expressed from tac promoterThis study
    pJK596pJAK16 with dspA of CU1000 expressed from tac promoterThis study
    pJK597pJAK16 with rmlA of CU1000 expressed from tac promoterThis study
  • a Kmr, kanamycin resistant; Cmr, chloramphenicol resistant.