TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescription or constructionSource or reference
Strains
    E. coli
        DH5αF′φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(r m+) deoR thi-1 supE44 λ gyrA96 relA112
        MT607pRK600 containing helper strain for conjugation25
        S17-1E. coli 294::RP4-2 (Tc::Mu)(Km::Tn7)26
        YB1329S17-1 λpir, pUT::mini-Tn5lacZ2M. R. K. Alley, unpublished
    C. crescentus
        CB15Wild type20
        CB2ArsaA (S-layer) mutant of CB228
        NA1000syn-1000, previously called NA1000; a derivative of CB15 that does not synthesize holdfasts6
        YB2861CB15 pleC100::mini-Tn5lacZ2This work
        YB2862CB15 hfsB101::mini-Tn5lacZ2This work
        YB2863CB15 podJ102::mini-Tn5lacZ2This work
        YB2864CB15 podJ103::mini-Tn5lacZ2This work
        YB2865CB15 hfsA106::mini-Tn5lacZ2This work
        YB2866CB15 podJ107::mini-Tn5lacZ2This work
        YB2867CB15 podJ108::mini-Tn5lacZ2This work
        YB2868CB15 pleC109::mini-Tn5lacZ2This work
        YB2869CB15 hfsA111::mini-Tn5lacZ2This work
        YB2870CB15 hfsA118::mini-Tn5lacZ2This work
        YB2877CB15 hfsB176::mini-Tn5lacZ2This work
        YB2878CB15 hfsA125::mini-Tn5lacZ2This work
        YB2879CB2A hfsD172::mini-Tn5lacZ2This work
        YB2779CB15 hfaB113::mini-Tn5lacZ2This work
        YB2780CB15 hfaB115::mini-Tn5lacZ2This work
        YB2781CB15 hfa-120::mini-Tn5lacZ2This work
        YB2782CB15 hfa-121::mini-Tn5lacZ2This work
        YB2783CB15 hfa-122::mini-Tn5lacZ2This work
        YB2784CB15 hfaB123::mini-Tn5lacZ2This work
        YB3738CB15 hfa-105::mini-Tn5lacZ2This work
        YB2833CB15 hfsA::pNPTShfsAThis work
        YB2837CB15 hfsB::pNPTShfsBThis work
        YB2841CB15 hfsC::pNPTShfsCThis work
        YB2845CB15 hfsD::pNPTShfsDThis work
        YB2832CB15 pNPTS138 hfsAThis work
        YB2836CB15 pNPTS138 hfsBThis work
        YB2840CB15 pNPTS138 hfsCThis work
        YB2845CB15 pNPTS138 hfsDThis work
        YB3136CB15N::pLW56 (also CMS27)34
Plasmids
    pRJ41Cosmid 4-1, a pLaFR5-derived cosmid containing the hfaABDC region of CB159
    placHfa74.64-kb PstI-HindIII fragment containing the hfa promoter through the middle of hfaC from pRJ41 was cloned into the PstI-HindIII sites of pRKlac290This work
    pNPTS138Litmus 38 derivative, oriT sacB KanrAlley, unpublished
    pNPTShfsApNPTS138 parent vector containing 500-bp fragments upstream and downstream of hfsAThis work
    pNPTShfsBpNPTS138 parent vector containing 500-bp fragments upstream and downstream of hfsBThis work
    pNPTShfsCpNPTS138 parent vector containing 500-bp fragments upstream and downstream of hfsCThis work
    pNPTShfsDpNPTS138 parent vector containing 500-bp fragments upstream and downstream of hfsDThis work