TABLE 1.

Strains and plasmids used in this study

Strain or plasmidGenotypeSource or reference
Strains
    DH5α E. coli φ80 lacZΔM15 deoR endA1 gyrA96 hsdR17 recA1 relA1 supE44 thi-1 Δ(lacZYA-argF)U169Life Technologies
    JM109 E. coli F′[traD36 proAB lacIqlacZΔM15] endA1 gyrA96 hsdR17 recA1 relA1 supE44 thi-1 Δ(lac-proAB) 43
    14028Wild-type S. enterica serovar TyphimuriumAmerican Type Culture Collection
    BA61214028 sdiA1::mTn3 1
    BA110114028 srgE5::MudJ 1
    BA110314028 srgA1::MudJ 1
    BA111014028 srg-6::MudJ 1
    BA111214028 srg-7::MudJ 1
    BA1301BA612 srgE5::MudJ 1
    BA1303BA612 srgA1::MudJ 1
    BA1310BA612 srg-6::MudJ 1
    BA1312BA612 srg-7::MudJ 1
    10460Wild-type Y. enterocolitica 37
    10460 yenI10460 yenI::Kan 37
    OSU239Wild-type H. alveiOhio State University Department of Microbiology culture collection
    PAO1Wild-type P. aeruginosa 16
    PAO-JP2PAO1 ΔlasI::tet ΔrhlI::Tn501-2 29
    97021Wild-type S. marcescensJean Guard-Petter
    CVOblu C. violaceum cviI::Tn5xylE 34
Plasmids
    pSB401 luxR + PluxI-luxCDABE Tetr p15A ori 40
    pSB536 ahyR + PahyI-luxCDABE Ampr ColE1 ori 34
    pSB1075 lasR + PlasI-luxCDABE Ampr ColE1 ori 40
    pBA428Prck-luxCDABE Tetr 22
    pJNS25PsrgE-luxCDABE TetrpSB401ΔEcoRI carrying the srgE-STM1555 intergenic region
    pJNS35PSTM1555-luxCDABE TetrSame as pJNS25 but the intergenic region is in the opposite orientation