TABLE 3.

Fermentation characteristicsa of strains for the asRNA downregulation of AADC in pH-controlled bioreactor experimentsb

StrainNo. of exptsGrowth rate (h−1)Maximum A600Acetone concnAcetone concn/maximum A600cButanol concnButanol concn/maximum A600cPeak acetate concn/maximum A600cFinal acetate concn/maximum A600cPeak butyrate concn/maximum A600cFinal butyrate concn/maximum A600cButanol/acetate ratio
ATCC 82420.47 ± 0.077.63 ± 0.3379 ± 410.4 ± 0.0139 ± 918.2 ± 0.09.8 ± 0.09.1 ± 0.211.3 ± 0.46.4 ± 0.61.75 ± 0.03
ATCC 824(SOS95del)60.45 ± 0.035.56 ± 0.4249 ± 29.5 ± 1.093 ± 317.3 ± 1.615.9 ± 1.814.7 ± 1.213.4 ± 1.110.3 ± 0.71.91 ± 0.08
ATCC 824(pADC38AS)20.43 ± 0.046.34 ± 0.0450 ± 17.9 ± 0.2118 ± 218.6 ± 1.817.2 ± 1.216.3 ± 0.911.9 ± 0.25.5 ± 1.82.34 ± 0.17
ATCC 824(pADC68AS)20.42 ± 0.025.56 ± 0.1947 ± 78.5 ± 0.9101 ± 1018.0 ± 1.117.4 ± 0.317.0 ± 0.514.2 ± 0.29.2 ± 0.72.14 ± 0.09
ATCC 824(pADC100AS)20.44 ± 0.066.43 ± 0.4273 ± 111.5 ± 0.9141 ± 122.0 ± 1.615.9 ± 0.414.5 ± 0.412.5 ± 0.57.0 ± 0.21.92 ± 0.01
  • a Mean ± standard error of the mean.

  • b Concentrations are millimolar.

  • c Ratios of product concentrations over the maximum cell density (A600) were calculated in order to normalize some key data for comparison purposes.