TABLE 4.

Fermentation characteristicsa of strains for the asRNA downregulation of CoAT in static-flask culturesb

StrainNo. of exptsMaximum A600Acetone concn/maximum A600cButanol concn/maximum A600cPeak acetate concn/maximum A600cFinal acetate concn/maximum A600cPeak butyrate concn/maximum A600cFinal butyrate concn/maximum A600c
ATCC 824(pSO595del)25.43 ± 0.367.9 ± 0.915.4 ± 1.55.1 ± 0.34.3 ± 0.45.6 ± 0.31.9 ± 0.4
ATCC 824(pCTFA2AS)24.26 ± 0.190.9 ± 0.014.8 ± 0.27.8 ± 0.58.2 ± 0.510.4 ± 0.610.4 ± 0.6
ATCC 824(pCTFB1AS)24.03 ± 0.000.0 ± 0.02.7 ± 0.38.0 ± 0.07.8 ± 0.111.9 ± 0.211.9 ± 0.2
ATCC 824(pCOAT11AS)24.28 ± 0.00.9 ± 0.54.6 ± 0.58.7 ± 0.15.9 ± 0.28.6 ± 0.48.4 ± 0.6

  • ↵a Mean ± standard error of the mean.

  • ↵b All concentrations are millimolar.

  • ↵c Ratios of product concentrations over the maximum cell density (A600) were calculated in order to normalize some key data for comparison purposes.