TABLE 2.

Oligonucleotides used for PCR

PrimerOligonucleotide sequencea (5′-3′)Use
ADCUPGTAAATATAAATAAATAGGACTAGAGGCGAUpstream primer for adc38, adc68, and adc100
ADC38DOWNCTGTCCGCTTTCTGGATCCCAADownstream primer for adc38
ADC68DOWNGCTTCATTAGCATCTAAGGCTDownstream primer for adc68
ADC100DOWNCACAGTTTAAAAAACCTCCAATCADownstream primer for adc100
CTFAUPATCTTAAATACTCGAGTTAAGAATTTUpstream primer for ctfa1 and ctfa2
CTFADOWN1GATCCGCCTGCTCGAGTTGGATCCACTAGAGDownstream primer for ctfa1
CTFADOWN2ATAGAAGGTGGATCCGGCCTCGAGTACAATDownstream primer for ctfa2
CTFBUPAGCAATGACCCTCGAGTTCTTATUpstream primer for ctfb1
CTFBDOWN1CGAAAAATGTGCCGTCTCGAGGATCCGTTGTDownstream primer for ctfb1
  • a Boldface letters in the oligonucleotide sequences indicate nucleotide substitutions used to generate useful restriction sites near the termini of the PCR products. Relevant restriction sites are underlined.