TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant featuresSource or reference
X. nematophila strains
    HGB007ATCC 19061, wild type, AmprATCC
    HGB081Spontaneous Rifr mutant of X. nematophila ATCC 19061S. Forst
    HGB340GFP+ derivative of HGB007, CmlrThis study
    HGB566Mini-Tn7-ECFP-containing derivative of HGB007, GmrThis study
    HGB567Mini-Tn7-DsRed gene-containing derivative of HGB007, GmrThis study
    STM-1HGB081 containing unique (probe A) signature-tagged mini-Tn513; this study
    STM-2HGB081 containing unique (probe B) signature-tagged mini-Tn513; this study
    STM-3HGB081 containing unique (probe C) signature-tagged mini-Tn513; this study
Plasmids
    pNK2859Contains mini-Tn10-kan, source of PaphA29
    pQBI63Source of Super-Glo GFP geneQbiogene
    pKR100oriR6K-based suicide vector, Cm1rK. Visick
    pKR100BpKR100 with unique BgIII siteThis study
    pECM9pQBI63 with PaphA in place of PT7This study
    pECM2PaphA-Super-Glo GFP gene fragment from pECM9 cloned into pKR100BThis study
    pECM20pECM2 containing a 614-bp chromosomal insert from ATCC 19061This study
    pBK-mini-Tn7(Gm)PA1/04/03-DsRed geneSource of mini-Tn7-PA1/04/03-DsRed gene, GmrL. Lambertsen
    pBK-mini-Tn7(Gm)PA1/04/03-ECFP geneSource of mini-Tn7-PAl/04/03-ECFP gene, GmrL. Lambertsen
    pUX-BF13Tn7 transposition helper plasmid (in E. coli S17-1 λ pir)5