TABLE 2.

CE-ES-MS and CE-ES-MS/MS data and proposed compositions of O-deacylated LPS from N. meningitidis strains H44/76 and H44/76::1638

StrainMS ions observed (m/z)MS/MS ions observed (m/z)Size of lipid A species (amu)aLipid A composition
3221
H44/769301,396475952952.262GlcN, 2 3-OH C14:0, 2P, H2O
9571,4365151,0321,032.242GlcN, 2 3-OH C14:0, 3P, H2O
9711,4575361,0741,075.312GlcN, 2 3-OH C14:0, 2P, PEtn, H2O
9981,4975771,1551,155.292GlcN, 2 3-OH C14:0, 3P, PEtn, H2O
1,0396381,278.342GlcN, 2 3-OH C14:0, 3P, 2PEtn, H2O
H44/76::16389301,396475952952.262GlcN, 2 3-OH C14:0, 2P, H2O
9571,4365151,0321,032.242GlcN, 2 3-OH C14:0, 3P, H2O
  • a Average mass units were used for calculation of molecular size based on proposed composition as follows: GlcN, 161.15 amu; 3-OH C14:0, 226.00 amu; P, 79.98 amu; PEtn, 123.05 amu; and H2O, 18.00 amu.