Purification of PhaZ2 from E. coli harboring phaZ2Reua

StepAmt of protein (mg)Activity (U)Sp act (U/mg)Yield (%)
Crude extract5001,4002.7(100)
Toyopearl DEAE-650M281,1003878
Toyopearl Butyl-650M145203838
Phenyl-Sepharose HP2.71806713
  • a Enzyme was purified from a 0.5-liter culture as described in the text. Dimer-hydrolyzing activity was measured enzymatically. One unit of the enzyme catalyzes the formation of 1 μmol of 3HB per min.