TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)aSource or reference
Strains
    E. coli
        JM109 recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′ [traD36 proAB+lacIqlacZΔM15]Takara
        BLR(DE3)/pLysSFompT hsdSB(rB mB) gal dcm Δ(srl-recA)306::Tn10(Tcr) (DE3)/pLysS(Cmr)Novagen
        S17-1 (λ pir)Tra+recA pro thi hsdR chr::RP4-2 25
    R. eutropha
        H16Wild typeATCC 17699
        H16-SK1544H16, phaZ1; 826-bp deletion mutant4
        D1ΔphaZ1 strain, derived from H16/pJpPH171; Kmr16
        OH1ΔphaZ2 strain, derived from H16/pJPOH; KmrThis study
        DO1ΔphaZ1 ΔphaZ2 strain, derived from H16-SK/pJPOH; KmrThis study
Plasmids
    pUC19Cloning vector; AprTakara
    pET23bExpression vector; AprNovagen
    pJP5603R6K-based suicide vector; Kmr15
    pAE171pUC with R. eutropha phaZ116
    pET171HpET23b carrying amplified NdeI-XhoI fragment containing His-tagged phaZ116
    pETOHpET23b carrying amplified NdeI-XhoI fragment containing phaZ2This study
    pJPPH171pJP5603 carrying PstI-HincII fragment of pAE17116
    pJPPOHpJP5603 carrying 306-bp PstI fragment of pETOHThis study
  • a Tcr, tetracycline resistant; Kmr, kanamycin resistant; Apr, ampicillin resistant; Cmr, chloramphenicol resistant.