TABLE 3.

Substrate specificity of PhaZ2a

SubstrateVmax (μmol/min/mg)Km (mM)Vmax/Km
Linear 3HB-oligomers
    Dimer780.35220
    Trimer590.33180
    Tetramer540.11490
    Pentamer500.15330
Cyclic 3HB-oligomers
    Trimer600.20300
    Pentamer590.14420
    Hexamer600.25240
Crystalline PHB granulesNDb
  • a V max and Km values were determined by pH stat.

  • b ND, not determined.