TABLE 1.

E. coli strains and plasmids used in this study

Strain or plasmidGenotypeaSource or reference
E. coli
    DH5αΔ(argF lac) U196 endA1 recA1 hsdR17(rK mK+) supE44 thi-1 gyrA1 relA1 (F′ φ80 ΔlacZΔM15)11
    AA93Δfec aroB Δ(argF lac)U169 araD139 rspL150 relA1 deoC1 flbB5301 ptsF25 rbsR22
    SU202lexA71::Tn5 sulA211 sulA(op408/op+)::lacZ Δ(lacIPOZYA)169/F′ lacIq lacZΔM15::Tn95
Plasmids
    pSV66pHSG576 fecI fecR fecA12
    pMS604ori ColE1 Tetr lexA1-87-fos zipper5
    pDP804ori p15A Ampr lexA1-87408-jun zipper5
    plexFecApDP804 lexA1-87408-fecA1-79This study
    plexANpMS604 lexA1-87This study
    plexRCpMS604 lexA1-87-fecR101-317This study
    plex237pMS604 lexA1-87-fecR237-317This study
    plex288pMS604 lexA1-87-fecR101-288This study
    plex278pMS604 lexA1-87-fecR101-278This study
    plexLZGpMS604 lexA1-87-fecR227-288This study
    pT7-7ori ColE1 phage T7 gene promoter28
    pAA70pT7-7 fecR34
    pLZ1ApT7-7 fecR(L247A)This study
    pLZ1CpT7-7 fecR(L247C)This study
    pLZ1pT7-7 fecR(L247P)This study
    pLZ2pT7-7 fecR(L254P)This study
    pLZ3pT7-7 fecR(L261P)This study
    pLZ4pT7-7 fecR(V268P)This study
    plexLZ1ApMS604 lexA1-87-fecR101-317(L247A)This study
    plexLZ1CpMS604 lexA1-87-fecR101-317(L247C)This study
    plexLZ1pMS604 lexA1-87-fecR101-317(L247P)This study
    plexLZ2pMS604 lexA1-87-fecR101-317(L254P)This study
    plexLZ3pMS604 lexA1-87-fecR101-317(L261P)This study
    plexLZ4pMS604 lexA1-87-fecR101-317(V268P)This study
    pFPV25ori ColE1 Ampr contains promoterless gfpmut330
    pGFPA′pFPV25 PfecA-gfpThis study
    pGFPAA′pFPV25 fecA PfecA-gfpThis study
    pHSG576pSC101 derivative, Cmr29
    pMMO203pHSG576 fecI fecR27
    pLCLZ1ApHSG576 fecI fecR(L247A)This study
    pLCLZ1CpHSG576 fecI fecR(L247C)This study
    pLCLZ1pHSG576 fecI fecR(L247P)This study
    pLCLZ2pHSG576 fecI fecR(L254P)This study
    pLCLZ3pHSG576 fecI fecR(L261P)This study
    pLCLZ4pHSG576 fecI fecR(V268P)This study
    pIS712pHSG576 fecI fecR fecA15
    pOR603pHSG576 fecI fecR(T2511C)(L254E) fecAThis study
    pOR601pHSG576 fecI fecR(C2255G/T2256C)(L269G) fecAThis study
    pOR600pHSG576 fecI fecR(T2600C/C2602G)(F284L) fecAThis study
    pOR603D1pHSG576 fecI fecR(T2511C)(L254E) fecA(C3054A)(G39R)This study
    pOR601D1pHSG576 fecI fecR(C2255G/T2256C) (L269G) fecA(C3054A) (G39R)This study
    pOR600D1pHSG576 fecI fecR(T2600C/C2602G) (F284L) fecA(C3054A) (G39R)This study
    pOR603C3pHSG576 fecI fecR(T2511C)(L254E) fecA(G3004C)(D43E)This study
    pOR601C3pHSG576 fecI fecR(C2255G/T2256C) (L269G) fecA(G3004C) (D43E)This study
    pOR600C3pHSG576 fecI fecR(T2600C/C2602G) (F284L) fecA(G3004C) (D43E)This study
    pORD1pHSG576 fecI fecR fecA(C3054A) (G39R)This study
    pORC3pHSG576 fecI fecR fecA(G3004C) (D43E)This study
    pLCIRApHSG576 fecI fecR fecA27
    pIS135pHSG576 fecI fecR22
    pHCIRpBCKS+ fecI fecR1-110This study
    pAS103pBCKS+ fecI fecR27
    pMS604fecA1-79pMS604 lexA1-87-fecA1-79This study
    pUS11pDP804 lexA1-87408-fecR101-3177
    pHBlexR2pDP804 lexA1-87408-fecR101-317 (T2164A/T2340C) (D138E/V197A)This study
    pHBlexR7pDP804 lexA1-87408-fecR101-317 (A2235G/A2471G/A2619T) (D162G/S241G/D290V)This study
    pHBlexR12pDP804 lexA1-87408-fecR101-317 (A2115G/T2142C/A2145G/A2232G/A2322G/A2504G/T2628A) (Q122R/L131P/N132S/K161R/N191S/K252E/L293Q)This study
    pHBlexR13pDP804 lexA1-87408-fecR101-317 (T2445C/G2548A) (V232A/G267S)This study
    pHBlexR15pDP804 lexA1-87408-fecR101-317 (A2423G/A2615G) (S225G/T289A)This study
    pHBlexR16pDP804 lexA1-87408-fecR101-317 (C2616A/T2649C) (T289N/L300P)This study
    pHBlexR21pDP804 lexA1-87408-fecR101-317 (A2212T/T2421C/A2524G/T2655C) (E154D/F224S/I258M/V302A)This study
    pHBlexR22pDP804 lexA1-87408-fecR101-317 (G2067T/A2244G) (G106V/Q165R)This study
    pHBlcR2pHSG576 fecI fecR (T2164A/T2340C) (D138E/V197A) fecAThis study
    pHBlcR7pHSG576 fecI fecR (A2235G/A2471G/A2619T) (D162G/S241G/D290V) fecAThis study
    pHBlcR12pHSG576 fecI fecR (A2115G/T2142C/A2145G/A2232G/A2322G/A2504G/T2628A) (Q122R/L131P/N132S/K161R/N191S/K252E/L293Q) fecAThis study
    pHBlcR13pHSG576 fecI fecR (T2445C/G2548A) (V232A/G267S) fecAThis study
    pHBlcR15pHSG576 fecI fecR (A2423G/A2615G) (S225G/T289A) fecAThis study
    pHBlcR16pHSG576 fecI fecR (C2616A/T2649C) (T289N/L300P) fecAThis study
    pHBlcR21pHSG576 fecI fecR (A2212T/T2421C/A2524G/T2655C) (E154D/F224S/I258M/V302A) fecAThis study
    pHBlcR22pHSG576 fecI fecR (G2067T/A2244G) (G106V/Q165R) fecAThis study
    pHBhcR2pBCKS+ fecI fecR (T2164A/T2340C) (D138E/V197A)This study
    pHBhcR7pBCKS+ fecI fecR (A2235G/A2471G/A2619T) (D162G/S241G/D290V)This study
    pHBhcR12pBCKS+ fecI fecR (A2115G/T2142C/A2145G/A2232G/A2322G/A2504G/T2628A) (Q122R/L131P/N132S/K161R/N191S/K252E/L293Q)This study
    pHBhcR13pBCKS+ fecI fecR (T2445C/G2548A) (V232A/G267S)This study
    pHBhcR15pBCKS+ fecI fecR (A2423G/A2615G) (S225G/T289A)This study
    pHBhcR16pBCKS+ fecI fecR (C2616A/T2649C) (T289N/L300P)This study
    pHBhcR21pBCKS+ fecI fecR (A2212T/T2421C/A2524G/T2655C) (E154D/F224S/I258M/V302A)This study
    pHBhcR22pBCKS+ fecI fecR (G2067T/A2244G) (G106V/Q165R)This study
  • a Subscripts denote amino acid residues of the encoded proteins. Numbers in parentheses indicate amino acids replaced.