TABLE 3.

Binding of mutated FecR101-317 to FecA1-79

PlasmidFecR proteinsβ-Galactosidase activitya (Miller units)
pSM604LexA1-87WT-Fos446
plexRCLexA1-87WT-FecR101-31736
plexLZ1ALexA1-87WT-FecR (L247A)54
plexLZ1CLexA1-87WT-FecR (L247C)94
plexLZ1LexA1-87WT-FecR (L247P)170
plexLZ2LexA1-87WT-FecR (L254P)367
plexLZ3LexA1-87WT-FecR (L261P)376
plexLZ4LexA1-87WT-FecR (L268P)112
  • a Determined by using the bacterial two-hybrid LexA-based system in E. coli SU202 sulA-lacZ, which, in addition to FecR derivatives, synthesized LexA1-87408-FecA1-79 encoded by plexFecA. WT, wild type.