TABLE 7.

Induction activity of FecR and FecA mutantsa

PlasmidProteinRelative fluorescence
NB mediumNB medium + citrate
pHSG576None4045
pIS712FecR FecA65856
pOR603FecR(L254E) FecA6770
pOR601FecR(L269G) FecA5966
pOR600FecR(F284L) FecA5863
pOR603D1FecR(L254E) FecA(G39R)6578
pOR601D1FecR(L269G) FecA(G39R)63318
pOR600D1FecR(F284L) FecA(G39R)48108
pOR603C3FecR(L254E) FecA(D43E)65139
pOR601C3FecR(L269G) FecA(D43E)65124
pOR600C3FecR(F284L) FecA(D43E)47289
pORD1FecR FecA(G39R)45801
pORC3FecR FecA(D43E)43764
  • a Induction was determined in E. coli AA93 Δfec(pGFPA′ fecA, PfecA::gfp). The plasmids listed encoded FecI in addition to the listed FecR and FecA derivatives.