TABLE 4.

Binding of mutated FecR101-317 to FecA1-79

PlasmidFecR proteinβ-Galactosidase activitya (Miller units)
pDP804LexA1-87408-Jun636
pUS11LexA1-87408-FecR101-317134
pHBLexR2LexA1-87408-FecR101-317 (D138E, V197A)93
pHBLexR7LexA1-87408-FecR101-317 (D162G, S241G, D290V)542
pHBLexR12LexA1-87408-FecR101-317 (Q122R, L131P, N132S, K161R, N191S, K252E, L293Q)607
pHBLexR13LexA1-87408-FecR101-317 (V232A, G267S)516
pHBLexR15LexA1-87408-FecR101-317 (S225G, T289A)537
pHBLexR16LexA1-87408-FecR101-317 (T289N, L300P)688
pHBLexR21LexA1-87408-FecR101-317 (E154D, F224S, I258M, V302A)754
pHBLexR22LexA1-87408-FecR101-317 (G106V, Q165R)731
  • a Determined by using the bacterial two-hybrid LexA-based system in E. coli SU202 sulA-lacZ, which, in addition to FecR derivatives, synthesized LexA1-87-FecR1-79 encoded by pMS604fecA1-79.