TABLE 5.

Induction activity of FecR leucine motif mutantsa

PlasmidFecR proteinRelative fluorescence
NB mediumNB medium + citrate
pHSG576None34
pMMO203FecR22356
pLCLZ1AFecR(L247A)21285
pLCLZ1CFecR(L247C)21235
pLCLZ1FecR(L247P)18107
pLCLZ2FecR(L254P)2528
pLCLZ3FecR(L261P)1036
pLCLZ4FecR(V268P)11174
  • a Induction was determined in E. coli AA93 Δfec(pGFPAA′ fecA, PfecA::gfp). The plasmids listed encoded FecI in addition to the listed FecR derivatives.