TABLE 3.

Quantitative analysis of nuclease, protease, and coagulase productiona

StrainProduction
ProteasebNucleasecCoagulased
Becker2.48 ± 0.370.490 ± 0.181100 ± 0
CYL56140.53 ± 0.920.632 ± 0.18014.8 ± 17.0
CYL10403.33 ± 0.330.013 ± 0.023100 ± 0
  • a All data are averages (± standard deviations) of three independent experiments.

  • b Protease production was analyzed by measuring depletion of 3 mg of azocasein/ml in 17 h, monitored by OD340.

  • c Nuclease production was analyzed by measuring depletion of 5 mg of salmon sperm DNA/ml monitored by OD260.

  • d Coagulase was measured by a tube coagulation test of threefold serial dilution samples. The numbers represent the highest dilution and are standardized by setting the activity of Becker as 100.