TABLE 2.

Interaction of truncated FecR101-317 derivatives with FecA1-79

PlasmidProteinsβ-Galactosidase activitya (Miller units)
pMS604LexA1-87WT-Fos478
plexRCLexA1-87WT-FecR101-31745
plex237LexA1-87WT-FecR237-31765
plex288LexA1-87WT-FecR101-288221
plex278LexA1-87WT-FecR101-278453
plexLZGLexA1-87WT-FecR227-288453
  • a Determined by using the bacterial two-hybrid LexA-based system in E. coli SU202 sulA-lacZ, which, in addition to FecR derivatives, synthesized LexA1-87408-FecA1-79 encoded by plexFecA. WT, wild type.