TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotypeReference or source
E. coli strains
    DH5αsupE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1BRLa
    S17-1thi pro hsdR hdsM+ recA, chromosomal insertion of RP4-2(Tc::Mu Km::T7)29
S. frigidimarina strains
    NCIMB400RWild type
    PSD1NCBIMB400R ifcA::ΩCmr5
    PSD106NCBIMB400R ifcR::ΩCmrThis study
    PSD201Complemented PSD106 carrying pPD1 Cmr, TcrThis study
    PSR33Complemented PSD106 carrying pFR33 Cmr, TcrThis study
Plasmids
    pBluescript II KS+Cloning vector, AmprStratagene
    pJQ200KSMobilizable suicide vector, Gmr26
    pRK415IncP broad-host-range cloning vector, Tcr13
    pHP45ΩCmCloning vector containing ΩCm, Ampr6
    pTRN12-kb XbaI-XhoI PCR fragment carrying ifcR cloned into pBluescript II KS+This study
    pTRN24-kb EcoRI fragment containing ΩCm cloned into pTRN1This study
    pTRN36-kb XbaI-ApaI fragment containing ifcR::ΩCmr cloned into pJQ200KSThis study
    pTCM16-kb XbaI fragment carrying ifcR, ifcO, and ifcA::ΩCmr cloned into pBluescript II KS+This study
    pPD11.3-kb XbaI-PstI fragment carrying ifcR plus 5′ and 3′ flanking sequences cloned into pRK415This study
    pFR3890-bp fragment carrying ifcR cloned into expression vector pT7-7This study
    pFR33932-bp fragment carrying a promotorless ifcR cloned into pRK415This study
  • a BRL, Bethesda Research Laboratories.