TABLE 1.

Plasmids used in this study

PlasmidFeature(s)Reference or Source
pBR322rep (pMB1) rop Apr Tcr3
pET-15boriR (ColE1) Apr, pT7, N-terminal His6 tagNovagen
pGS-lac-02pAB-1 with a promoterless lacZ gene16
pGS-reg-RP1pOG-R125 containing a mutation in the promoter of icmRThis study
pGS-reg-RP2pOG-R125 containing a mutation in the promoter of icmRThis study
pLAW344sacB MCSa oriT (RK2) Cmr oriR (ColE1) Apr47
pMMB207RSF1010 derivative, IncQ Cmr oriT MCS29
pOG-BCC2E. coli cpxR in pOG-BRR3This study
pOG-BRM1pOG-R125 containing a mutation in the BRM1 site of icmRThis study
pOG-BRM2pOG-R125 containing a mutation in the BRM2 site of icmRThis study
pOG-BRR1icmR::lacZ fusion from pOG-R-125 in pBR322This study
pOG-BRR2pOG-BRR1 containing a single EcoRI siteThis study
pOG-BRR3Ptac promoter in pOG-BRR2This study
pOG-cpxA-Km1pOG-U-cpxRA with the Kmr cassette in cpxAThis study
pOG-cpxA-Km2Insert of pOG-cpxA-Km1 in pLAW344This study
pOG-cpx-C2E. coli cpxR in pUC18This study
pOG-cpxR-1L. pneumophila cpxR in pUC18This study
pOG-cpxR-2pOG-cpxR-1 with the Kmr cassette in cpxRThis study
pOG-cpxR-3Insert of pOG-cpxR-2 in pLAW344This study
pOG-ECP2L. pneumophila cpxR in pET-15bThis study
pOG-R-125icmR::lacZ fusion in pGS-lac-0150
pOG-R-cpx3L. pneumophila cpxR in pOG-R125This study
pOG-RCPR3L. pneumophila cpxR in pOG-BRR3This study
pOG-R-cpxRAL. pneumophila cpxRA in pOG-R125This study
pOG-rpoE-1L. pneumophila rpoE in pUC18This study
pOG-rpoE-2pOG-rpoE-1 with the Kmr cassette in rpoEThis study
pOG-rpoE-3Insert of pOG-rpoE-2 in pLAW344This study
pOG-RR16L. pneumophila genomic insert in pOG-BRR3This study
pOG-U-cpxRAL. pneumophila cpxRA in pUC18This study
pOG-U-ECPL. pneumophila cpxR in pUC18This study
pSS-R27Regulatory region of icmR in pMC140350
pUC18oriR (ColE1) MCS Apr49
  • a MCS, multiple cloning site.