TABLE 4.

Efficiency of sporulation of phrA deletion and insertion strainsa

StrainbRelevant genotypeViable cell count ml−1Spore count ml−1Percentagef
JH642Wild type3.1 × 1081.6 × 10851.6
JH11842ΔphrA2.8 × 1081.2 × 1074.3
JH23053c phrA (wild type)2.0 × 1081.1 × 10855.0
JH23054c phrAΔ143.1 × 1089.8 × 10731.6
JH23055c phrAΔ14-64.5 × 1081.3 × 10828.9
JH23056c phrA-(His codon)66.4 × 1071.3 × 10720.3
JH23008d, eΔphrA amyE::phrA (wild type)9.4 × 1075.7 × 10760.6
JH11831d amyE::phrAΔ382.4 × 1081.4 × 10858.3
JH11847d, eΔphrA amyE::phrAΔ382.3 × 1085.8 × 10725.2
JH23007d, eΔphrA amyE::phrAΔ213.9 × 1081.3 × 10833.3
  • a Data are representative of results from three independent experiments.

  • b Strains were grown for 24 h at 37°C in Schaeffer's sporulation medium.

  • c The phrA construct is integrated isotopically by single crossover.

  • d The phrA construct is integrated ectopically (amyE) by double crossover.

  • e Strains are derivatives of JH11842.

  • f The percentage is calculated as the ratio between spore count and viable cell count.