TABLE 4.

Specific CoA ligase activities of T. aromatica acting on benzoate and 2-aminobenzoatea

Growth substrateActivity [nmol min−1 (mg of protein)−1)] of:
Benzoate-CoA ligase2-Aminobenzoate-CoA ligase
2-Aminobenzoate4536
Benzoate11550
Phenol10964
4-Hydroxybenzoate7838
3-Hydroxybenzoate6032
Toluene12686
Phenylacetate11474
Acetate5828
Glutarate3622
Benzoate + oxygen8936
  • a Cells grown under denitrifying conditions with different aromatic substrates were tested. The cell extracts were precipitated with ammonium sulfate (55% saturation) prior to measurement of the enzyme activities in the dissolved precipitate. The specific activity refers to mg of protein in cell extract (supernatant from centrifugation at 100,000 × g). The values given are the average of at least two parallel measurements with less than 10% deviation.