TABLE 1.

Diol dehydratase activity during organelle purification

SampleProtein (mg)Activity (μmol/min)aSp act (μmol/min/mg)aYield (%)Purification (fold)
Crude extract5001,3462.71001
Detergent/salts treatment370.61,0002.774.31
Supernatant (12,000 × g)3575731.642.60.6
Pellet (48,000 × g)9.419420.814.47.7
Supernatant (12,000 × g)719527.914.510.3
Sucrose density gradient0.61627.51.210.2
  • a Activities and specific activities were determined by measuring the amount of propionaldehyde formed using the assay as described in Materials and Methods.