TABLE 2.

Anaerobic fdnG-lacZ expression in batch and continuous cultures in response to nitrate, nitrite, and formate availabilitya

StrainBatch expression with the following additions:Chemostat expression with the following additions:
NoneNO3NO2HCOOHNoneNO3NO2HCOOH
Wild type3053,0304704101765,000318295
narP2953,110465NDb2004,390220ND
narL285535285ND175520320ND
  • a Cells were grown on glucose minimal medium anaerobically as described in Materials and Methods. Where indicated, sodium nitrate (initial concentration, 40 mM), nitrite (5 mM), or formate (30 mM) was added. Units are given as nanomoles of ONPG hydrolyzed per minute per milligram of protein.

  • b ND, not determined.