TABLE 1.

Strains and plasmids

Strain or plasmidGenotypea or relevant characteristicsDeletion or cloned end pointsbSource or referencec
Strains
    14028Wild-type serovar TyphimuriumATCCd
    JS135zii-8104::Tn10dTc78
    JS247Φ(tetA+-rtsA+B+)34561769-4561768
    JS248ΔrtsA54561755-4560884
    JS249ΔrtsB64560868-4560602
    JS250ΔrtsAB74561769-4560602
    JS251ΔhilA112::Cm3019885-3021480
    JS252ΔhilC113::Cm3012135-3012976
    JS253ΔhilD114::Cm3017865-3018730
    JS254ΔinvF100::Cm3043931-3043290
    JS255ΔhilC-A2914::Cm3012135-3021480
    JS256ΔhilC-D2915::Cm3012135-3018730
    JS257ΔslrP100::Cm866973-869216
    JS258Φ(tetRA-rtsA+B+)3 PhilD111::MudJ
    JS259Φ(tetRA-rtsA+B+)3 invC101::MudJ
    JS260Φ(tetRA-rtsA+B+)3 sopA100::MudJ
    JS261Φ(tetRA-rtsA+B+)3 sopB101::MudJ
    JS262Φ(tetRA-rtsA+B+)3 icgA1::MudJ
    JS263Φ(tetRA-rtsA+B+)3 yhgF:231:MudJ
    JS264zii-8104::Tn10dTc PhilD111::MudJ
    JS265zii-8104::Tn10dTc invC101::MudJ
    JS266zii-8104::Tn10dTc sopA100::MudJ
    JS267zii-8104::Tn10dTc sopB101::MudJ
    JS268zii-8104::Tn10dTc icgA1::MudJ
    JS269zii-8104::Tn10dTc yhgF231::MudJ
    JS270ΔrtsAB7 ΔhilA112::Cm
    JS271ΔrtsAB7 invC101::MudJ
    JS272ΔrtsAB7 ΔhiD114::Cm
    JS273ΔrtsAB7 Φ(hilD-lac+)114
    JS274ΔrtsAB7 Φ(hilC-lac+)113
    JS275ΔrtsAB7 Φ(hilA-lac+)112
    JS276ΔrtsAB7 Φ(invF-lac+)100
    JS277ΔrtsAB7 flhC5456::MudJ
    JS278ΔrtsAB7 fliC5050::MudJ
    JS279Φ(hilA-lac+)112
    JS280ΔrtsA5 Φ(hilA-lac+)112
    JS281ΔrtsB6 Φ(hilA-lac+)112
    JS282Φ(invF-lac+)100
    JS283ΔrtsA5 Φ(invF-lac+)100
    JS284ΔrtsB6 Φ(invF-lac+)100
    JS285sopA100::MudJ
    JS286ΔrtsA5 sopA100::MudJ
    JS287ΔrtsB6 sopA100::MudJ
    JS288ΔrtsAB7 sopA100::MudJ
    JS289sopB101::MudJ
    JS290ΔrtsA5 sopB101::MudJ
    JS291ΔrtsB6 sopB101::MudJ
    JS292ΔrtsAB7 sopB101::MudJ
    JS293invC101::MudJ
    JS294ΔrtsA5 invC101::MudJ
    JS295ΔrtsB6 invC101::MudJ
    JS296icgA1::MudJ
    JS297ΔrtsA5 icgA1::MudJ
    JS298ΔrtsB6 icgA1::MudJ
    JS299ΔrtsAB7 icgA1::MudJ
    JS300ΔrtsAB7 ΔhilC113::Cm Φ(hilA-lac+)112
    JS301ΔrtsAB7 ΔhiD114::Cm Φ(hilA-lac+)112
    JS302ΔrtsAB7 ΔhilC-D2915::Cm Φ(hilA-lac+)112
    JS303ΔrtsAB7 ΔhilA112::Cm Φ(invF-lac+)100
    JS304ΔrtsAB7 ΔhilC-D2915::Cm Φ(invF-lac+)100
    JS305ΔrtsAB7 ΔhilC-A2914::Cm Φ(invF-lac+)100
    JS306ΔrtsAB7 sopA100::MudJ
    JS307ΔrtsAB7 ΔhilA112::Cm sopA100::MudJ
    JS308ΔrtsAB7 ΔinvF100::Cm sopA100::MudJ
    JS309ΔrtsAB7 sopB101::MudJ
    JS310ΔrtsAB7 ΔhilA112::Cm sopB101::MudJ
    JS311ΔrtsAB7 ΔinvF100::Cm sopB101::MudJ/PICK>
    JS312ΔrtsAB7 invC101::MudJ
    JS313ΔrtsAB7 ΔhilA112::Cm invC101::MudJ
    JS314ΔrtsAB7 ΔinvF100::Cm invC101::MudJ
    JS315ΔrtsAB7 icgA1::MudJ
    JS316ΔrtsAB7 ΔhilA112::Cm icgA1::MudJ
    JS317ΔrtsAB7 ΔinvF100::Cm icgA1::MudJ
    JS318ΔrtsAB7 Φ(slrP-lac+)100
    JS319ΔrtsAB7 ΔhilC-D2915::Cm Φ(slrP-lac+)100
    JS320ΔrtsAB7 ΔinvF100::Cm Φ(slrP-lac+)100
    JS321ΔrtsAB7 ΔhilA112::Cm Φ(slrP-lac+)100
    JS322ΔrtsA5 ΔhilC-D2915::Cm Φ(hilA-lac+)112
    JS323ΔrtsA5 ΔhilC-D2915::Cm Φ(slrP-lac+)100
    JS324Φ(rtsA-lac+)5
    JS325Φ(rtsB-lac+)6
    TT10286LT2 hisD9953::MudJ his-9944::MudI40
    TH1077LT2 fliC5050::MudJ34
    TH4054LT2 flhC5456::MudJ18
    DH5αλpirE. coli K-12 endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lac-argF)U169 deoR φ80 Δ(lac)M15 λpir+Lab stock
    MG1655E. coli K-12 F λ ilvG rfb-50 rph-1Lab stock
Plasmids
    pKD46bla PBAD gam bet exo pSC101 oriTS23
    pCP20bla cat cI857 λPR flp pSC101 oriTS15
    pKD3bla FRT cat FRT PS1 PS2 oriR6K23
    pKD13bla FRT ahp FRT PS1 PS4 oriR6K (for creating in-frame deletions)23
    pCE36ahp FRT lacZY+ this oriR6K27
    pCE37ahp FRT lacZY+ this oriR6K27
    pBAD30bla araC PBAD pACYC184 ori36
    pCE46bla araC λAttL1 ccdB+ cat λAttL2 pACYC184 ori
    pRtsAbla PBAD λAttB1 rtsA+ λAttB2 pACYC184 ori4561766-4560885
    pRtsBbla PBAD λAttB1 rtsB+ λAttB2 pACYC184 ori4560890-4560595
    pRtsABbla PBAD λAttB1 rtsA+B+ λAttB2 pACYC184 ori4561766-4560595
    pCE81bla PBAD λAttB1 myc-rtsA λAttB2 pACYC184 ori4561737-4560885
    pCE82bla PBAD λAttB1 myc-rtsB λAttB2 pACYC184 ori4560850-4560595
    pLS118bla PBAD hilD-myc-His pACYC184 ori72
    pLS119bla PBAD hilC-myc-His pACYC184 ori72
  • a Unless otherwise noted, all strains are isogenic derivatives of 14028.

  • b Numbers indicate the base pairs (inclusive) that are deleted (for strains) or cloned (for plasmids), as defined in the S. enterica serovar Typhimurium LT2 genome sequence in the National Center for Biotechnology Information database.

  • c This study, unless otherwise noted.

  • d ATCC, American Type Culture Collection.