TABLE 3.

Results of peptide mass fingerprintinga

Protein analyzedIdentity assignedNCBI accession no.MS-Fit analysisProfound analysis
No. of peptides matchedMOWSE score% Sequence coverageZ score% Sequence coverage
1PduG16765373155.657E+07292.3340
2PduC2587029174.674E+07312.4331
3PduC1676537061.951E+03162.4352
4PduP16765381689.1151.8216
5PduP16765381151.622E+05392.3347
6PduP16765381151.3E+07362.2754
7PduO16765380103.331E+06432.3552
8PduD1676537171.371E+04282.4322
9PduB2587033263.567E+04472.4344
10ND
11PduB′1676536983.43E+03432.0344
12ND
13PduK1676537662.42E+03531.7439
14PduK1676537662.42E+03532.1239
15PduT1676538492.209E+05422.4339
16ND
17NS
18PduU16760987-161.262E+08631.7463
19PduH167653742.4328
20PduJ1676097882.24E+05782.3378
21ND
22ND
  • a The numbers in column 1 refer to the protein spots similarly numbered in Fig. 4. Both the MS-Fit and Profound programs were used to compare the MALDI-TOF MS data obtained for organelle proteins to predicted spectra for proteins present in the GenBank and Swiss-Prot databases. A match was considered significant if the MOWSE score was at least 1e+003 (MS-Fit) or the Z score was at least 1.65 (Profound), and the sequence coverage was at least 25%. Abbreviations: ND, not determined; NS, no significant matches.