TABLE 1.

Bacterial strains and plasmids

Strain or plasmidGenotype or descriptionSource or reference
E. coli K-12
    MC1061hsdR hsdM araD139 Δ(ara-leu)7679 Δlac(IPOZYA) galU galK rpsL34
    CCI18ΔphoA20 galE galK thi rpsE rpoB recA1 argE(Am) ΔlacX7418
    HW1273CC118 harboring pHW127314
Plasmids
    pHW735pJK282 + BamHI fragment carryng most of ipaB::Tn3-lac of pHB76, Δ(invE virF)Kmr37
    pHW749Both invE and virF cloned into pHSG 415, Cmr37
    pHW1273Large virulence plasmid pSS120 of S. sonnei HW383 carrying Tn1, Apr14
    pKD46::Cmλ Red helper plasmid:oriR101 repA101(Ts)P-araB-gam-bet-exo CmrThis study
    pKD13Kmr gene with FRT sequence7
    pCP20FLP helper plasmid; pSC101 replicon (Ts) bla+ cat+ Flp(λRp) c1857 Apr Cmr7
    pTT0606pHW1273 with region upstream of icsB promote deleted from −1740 to −136 (using primers H2 and H5 for disruption; see Table 2)This study
    pTT0818pHW1273 with region upstream of icsB promoter deleted from −1740 to −41 (using primers H2 and H6 for disruption; see Table 2)This study
    pTT1405pHW1273 with region upstream of icsB promoter deleted from −1740 to −74 (using the primers H2 and H10 for disruption; see Table 2)This study
    pTT1608pHW1273 with region upstream of icsB promoter deleted from −1740 to −84 (using primers H2 and H11 for disruption; see Table 2)This study
    pTT1804pHW1273 with region upstream of icsB promoter deleted from −1740 to −94 (using primers H2 and H12 for disruption; see Table 2)This study
    pTT2002pHW1273 with region upstream of icsB promoter deleted from −1740 to −104 (using primers H2 and H13 for disruption; see Table 2)This study
    pTT2201pHW1273 with region upstream of icsB promoter deleted from −1740 to −85 (using primers H2 and H13-1 for disruption; see Table 2)This study
    pTT2202pHW1273 with region upstream of icsB promoter deleted from −1740 to −86 (using primers H2 and H13-2 for disruption; see Table 2)This study
    pTT2203pHW1273 with region upstream of icsB promoter deleted from −1740 to −87 (using primers H2 and H13-3 for disruption; see Table 2)This study
    pGEX-5X-3Expression vector, tacP lacIq Sj26 Apr33
    pQW2001pGEX-5X-3 + a fragment encoding full-length of InvE protein, AprThis study