TABLE 4.

Molecular mass, pI, and densitometry analyses

ProteinMolecular mass (kDa)pI% Total proteinMolar ratio
ObservedCalculatedaObservedbCalculateda
PduG68.864.55.55.55.42
PduC65.360.34.8-5520.88.5
PduP52.949.06.0-6.46.384
PduO36.336.86.67.33.62.5
PduB25.628.04.8-5.35.212.811.5
PduD25.624.26.9c8.94.85
PduB′23.024.04.8-5.75.612.112.5
PduK16.416.84.9-5.35.21.62.5
PduT16.419.15.55.91.62
PduE16.419.15.85.77.910.3
Spot179.0NDd5.7ND0.8ND
PduU8.612.566.11.53
PduH8.613.46.870.61
PduA7.79.66.9c7.47.519.5
PduJ7.5-7.79.05.8-6.46.51130.5
  • a Calculated from the DNA sequence.

  • b The isoelectric point of each protein was determined from the gel pictured in Fig. 4. Both the molecular masses and the relative protein amounts were calculated from a similar gel supplemented with dodecyl-maltoside to reduce streaking and stained with Sypro Ruby, which has an extended linear range (6). In cases where multiple protein spots corresponded to a single protein, a range is given for the observed pI.

  • c The pI values of PduA and PduD could not be accurately determined experimentally because both proteins focused at the upper limit of the pH range for the gel employed for pI determination (pH 7) and both have calculated pI values above 7.

  • d ND, not determined.