Morphological characterization of S. enterica serovar enteritidis 3b and isogenic agfA or agfB mutants

StrainaColony colorbMorphologycS. enterica serovar Typhimurium morphologydCR bound (μg)e
3b (agfBA+)RedAg(++)rdar35.8 ± 9.9
    ΔbcsARed-brownAg(++)bdar44.1 ± 12.3
    galE::Tn10Orange-brownAg(+)N/Af34.7 ± 6.1
    ΔbcsA galE::Tn10Orange-brownAg(+)N/A34.5 ± 2.9
ΔagfA mutantPinkNAgpdar5.6 ± 0.6
    ΔbcsAWhiteNAgsaw3.7 ± 0.4
    galE::Tn10Pink-orangeNAgN/A8.4 ± 1.5
    ΔbcsA galE::Tn10PinkNAgN/A7.3 ± 1.5
ΔagfB mutantPinkNAgpdar4.6 ± 0.6
    ΔbcsAWhiteNAgsaw3.6 ± 0.6
    galE::Tn10Pink-orangeNAgN/A11.2 ± 2.2
    ΔbcsA galE::Tn10PinkNAgN/A10.6 ± 1.1
  • a S. enteritidis strains as described in Materials and Methods.

  • b Color of colonies judged after growth on TCR agar for 24 h at 37°C.

  • c The morphology of colonies was compared to that of the 3b parent strain Ag(++), most aggregative (colonies dry and difficult to break apart); Ag(+), aggregative (colonies dry but easier to break apart); NAg, nonaggregative (colonies mucoid).

  • d Colony phenotypes of analogous S. enterica serovar Typhimurium strains grown on Luria agar without salt (containing 40 μg of CR per ml and 20 μg of Coomassie brilliant blue per ml) rdar, red, dry, and rough; bdar, brown, dry, and rough; pdar, pink, dry, and rough; saw, smooth and white. Phenotypes are as reported previously (35, 36, 48).

  • e Amount of CR eluted from 10 A600 units of cells ± standard error of the mean from four independent experiments.

  • f N/A, not applicable.