TABLE 2.

Bacterial strains and plasmids used in this study

Plasmid or strainRelevant characteristicsSource or reference
Plasmids
    pRK600Conjugal transfer helper, Cm9
    pRK609pRK600 with TnphoA, Cm, Nm22
    pVO155Vector used for transcriptional fusions to uidA; Ap Nm30
    pDKR50pVO155 derivative, SMb21243D. H. Keating (unpublished data)
    pDW110pVO155 derivative, nodF37
    pDW187pVO155 derivative, rrnCThis work
Strains
    Rm1021S. meliloti wild type, Str25
    DW186ΔrelA Str38
    DW362ΔrelA (L792V) rpoC StrThis work
    DW364ΔrelA (D453Y) rpoB StrThis work
    DW366ΔrelA (F620V) rpoC StrThis work
    DW371ΔrelA Δ458-460 (LGN) rpoB StrThis work
    DW386Rm1021, integrated pDW110, Str Nm37
    DW387DW186, integrated pDW110, Str NmThis work
    DW527Rm1021, integrated pDKR50, Str NmThis work
    DW528DW186, integrated pDKR50, Str NmThis work
    DW529DW362, integrated pDKR50, Str NmThis work
    DW530DW364, integrated pDKR50, Str NmThis work
    DW531DW366, integrated pDKR50, Str NmThis work
    DW532DW371, integrated pDKR50, Str NmThis work
    DW564Rm1021, integrated pDW187, Str NmThis work
    DW565DW186, integrated pDW187, Str NmThis work
    DW566DW362, integrated pDW187, Str NmThis work
    DW567DW364, integrated pDW187, Str NmThis work
    DW568DW366, integrated pDW187, Str NmThis work
    DW569DW371, integrated pDW187, Str NmThis work
    DW576DW362, integrated pDW110, Str NmThis work
    DW577DW364, integrated pDW110, Str NmThis work
    DW578DW366, integrated pDW110, Str NmThis work
    DW579DW371, integrated pDW110, Str NmThis work