TABLE 1.

E. coli strains and plasmids used

Strain or plasmidGenotype or phenotypeReference or source
Strains
    BL21(DE3) fhuAF hsdSB(rB mB) gal dcm ompT λ(DE3); T7 polymerase under lacUV5 control; fhuA43 and this study
    H1605mcrA hsdR thr ser rpsL aroB zhe-4::Tn10 lacK. Hantke
    AB2847aroB tsx malT thi18
    41/2AB2847 cir fepA fhuA18
    HK99AB2847 tonB fhuA26
    BR158AB2847 tonB24
    H1857AB2847 fhuACDBK. Hantke
    CH1857AB2847 fhuACDB tonBK. Hantke
    MB97AB2847 ΔfhuAThis study
    MB99BR158 ΔfhuAThis study
    MB21BL21(DE3) ΔfhuAThis study
Plasmids
    pT7-6Ampr; ColE1 origin44
    pT7-7Ampr; ColE1 origin44
    pHSG576Cmr; pSC101 origin45
    pSUKSCmr; p15A originR. Schönherr
    pHK763pT7-6 fhuA wild type24
    pABpT7-6 fhuA with BamHI E159D27
    pBK7pT7-6 fhuAΔ5-160 E3D3
    pHK570pHSG576 fhuA wild type28
    pBK570pHSG576 fhuAΔ5-160 E3D28
    p76SH15pT7-6 fhuA V11D13
    pFhuA517pT7-6 fhuAΔ5-17 E3G13
    pFE18pT7-6 fhuA E522R13
    pFE22pT7-6 fhuA E571R13
    pFE60pT7-6 fhuA T27C P533C13
    pCEc761pT7-6 fhuA 1-160 E159DThis study
    pCEc762pT7-6 fhuA 1-160 Δ5-17 E3G E159DThis study
    pCEcSU1pSUKS fhuA 1-160 E159DThis study
    pCEc771pT7-7 fhuA 1-160 E159DThis study
    pCEc772pT7-7 fhuA 1-160 Δ5-17 E3G E159DThis study
    pCEc773pT7-7 fhuA 1-160 E3D; without its signal sequenceThis study
    pCEcS77pT7-7 fhuAΔ5-160 E3D; without its signal sequenceThis study
    pMBlc1pHSG576 fhuA 1-160 E159D fhuAΔ5-160 E3D; under control of PT7 gene 10This study
    pMBlc2pHSG576 fhuA 1-160 Δ5-17 E3G E159D fhuAΔ5-160 E3D; under control of PT7 gene 10This study
    pMBlc3pHSG576 fhuA 1-160 E159D fhuA wild type; under control of PT7 gene 10This study
    pMBlc4pHSG576 fhuA 1-160Δ5-17 E3G E159D fhuA wild type; under control of PT7 gene 10This study
    pMBlc5pHSG576 fhuA 1-160 T27C E159D fhuAΔ5-160 E3D P533C; under control of PT7 gene 10This study
    pMBlc6pHSG576 fhuA 1-160 Δ5-17 E3G E159D T27C fhuAΔ5-160 E3D P533C; under control of PT7 gene 10This study
    pMBlc7pHSG576 fhuA 1-357; under control of PT7 gene 10This study
    pMBLc8pHSG576 fhuA 1-160 E159D; under control of PT7 gene 10This study
    pMBLc9pHSG576 fhuA 1-160 Δ5-17 E3G E159D; under control of PT7 gene 10This study
    pMBFE1pHSG576 fhuA T27C P533C; under control of PT7 gene 10This study
    pMBFE2pHSG576 fhuAΔ5-17 T27C E3G P533C; under control of PT7 gene 10This study
    pTO4pBR322 cma cmi36
    pTUC203pACYC184 mcjABCD42