TABLE 1.

Conservation of binding-site residues among RND multidrug efflux pumps

PumpResidue
Phe386Phe388Phe458Phe459Leu25Lys29Asp99Asp101
AcrBPhePhePhePheLeuLysAspAsp
AcrFPheTyrPhePheLeuGlnAspAsp
AcrDPheTyrPhePheLeuSerAspAsp
YhiVValPhePheMetLeuAsnSerAsp
MexBPhePhePhePheLeuSerAspAsp
MexDLeuPhePhePheLeuLysAspAsp
MexFLeuPhePhePheIleGlnAspAsp
MexYLeuPhePheIleLeuPheAsnAsp
CusAaGlnLeuThrLeuTrpAsnAspTyr
  • a The CusA (YdbE) protein, an E. coli RND transporter that is involved in copper ion efflux (10), was included for comparison.