TABLE 1.

Luciferase activity expressed by mutant DNA fragments.

PlasmidaLuciferase RLU (103)b
Without H2O2With H2O2
pMYT101869 ± 1761,080 ± 202
pMYT1021,682 ± 3701,638 ± 332
pMYT1039,528 ± 1,0548,549 ± 1,314
pMYT1049,632 ± 2,5669,616 ± 2,844
  • a The plasmids were derived from pSG10ter by insertion of the M. tuberculosis −154/+33 fragments upstream of the luxAB reporter gene. pMYT101 carries the wild-type sequence, and pMYT102, pMYT103, and pMYT104 carry the B, C, and D mutant sequences indicated in Fig. 6, respectively.

  • b Luciferase activity was measured as described in the legend to Fig. 1. The cultures were treated with 0.062 mM H2O2 for 1 h. The mean values ± standard deviations for four independent clones tested are reported.