TABLE 2.

Purification of pMMO from M. capsulatus Bath using duroquinol, NADH, NADH plus duroquinol or duroquinol alone, NADH, and NDH as the reductantsa

FractionCopper concn (nmol/mg of protein)Iron concn (nmol/mg of protein)Propylene oxidation rate (nmol · min−1 · mg of protein−1)
FormateDuroquinolNADHDuroquinol + NADHDuroquinol + NADH + NDH
Whole cell121 ± 2121 ± 8235 ± 68NDbNDNDND
Washed membrane237 ± 4254 ± 13ND63 ± 2381 ± 3778 ± 45ND
Dodecyl β-d-maltoside extract193 ± 2346 ± 16ND51 ± 2725 ± 1474 ± 21ND
First DEAE-Sepharose FF117 ± 1416 ± 3ND73 ± 3436 ± 13101 ± 33147 ± 43
Second DEAE-Sepharose FF101 ± 1618 ± 5ND95 ± 31097 ± 32134 ± 36
  • a Values represent results from four different preparations.

  • b ND, not determined.