TABLE 1.

Bivariate correlations of copper concentration in the culture media to methane oxidation activity and concentrations of copper, pMMO peptide and transcript, and fatty acids per cell

Measurement and fractionCopper concn (μM) with copper additiona:
0-80 μM0-59 μM60-80 μM
Methane oxidation
    Whole cell0.91**0.91**0.416
    Soluble−0.342−0.382ND
    Membrane0.959**0.921**0.957**
Copper
    Spent medium0.754*0.6440.898**
    Whole cell0.766**0.848**−0.937
    Soluble0.902**0.787*0.983*
    Membrane0.846**0.867**−0.258
Peptide
    sMMO−0.78−0.83ND
    pMMO0.93*0.980**0.673
Transcript
    sMMO−0.568−0.568ND
    pMMO0.977*0.977*ND
Fatty acids
    14:000.871**0.911**−0.811
    16:1 cis90.6260.898**−0.917
    16:1 cis110.911**0.838**−0.473
    16:000.695*0.872**−0.993
    17:000.742*0.437−0.132
    16:0 3OH0.6160.906**−0.955
    15:000.549**0.670−0.948
    Total0.710*0.870**−0.973
  • a *, P ≤ 0.05; **, P ≤ 0.01. ND, not determined.