TABLE 2.

Primers used in this study

PrimerSequenceaSiteb
Eryt-UP GCAAACTgcAGAGTGTGTTGATAG PstI
Eryt-DO CCGTAGGCGCTcGaGACCTCTTTAGCTTCTTG XhoI
Ery-DO-C CAGGTTACTAAAGGGAATGGAG
Fla-UP-LGGTTCCGTACTAgTTGTACTGTCTGC SpeI
Fla-UP-RaatttaactcgagCCACCAATCCCACACCACCCTTACC XhoI
Fla-DO-LATAAATGTAAAGGaTCCGTTTCATAGCTAAGG BamHI
Fla-DO-RCGCCGTCAACCCgGgTGCCGAGCTGGAAAAAGAGC XmaI
Fla-UP-CCTGAAGCAAAGTCGGAAAACGCCGGC
Fla-DO-C2CTCGAAACCGGTTCTGACGG
FurP-1cggatgaattcTCACGGAAATGCCTTTCTGTGC EcoRI
FurP-2attcagggatccCTTCCGCATGCGTCTCGAAC BamHI
Fmb-FcggatccatATGGAAAAATTCAACAATATTGCAC NdeI
Fmb-RattcagggatccTTAACGTTTGCCCTTGGCCTG BamHI
Fur-NattcaggatgcatTCACGGAAATGCCTTTCTGTGC NsiI
FkoB-1attcaggaattcGAGCGGTGTCATGTGTGTTCC EcoRI
FkoB-2attcagggatccGACGTTATAATACGCAATTTCGGCC BamHI
FkoB-3attcagggatccCCGGACGGTTTGTTGTTCAGAC BamHI
FkoB-4attcagctgcagGAATGCGCGTACCCCATTTCG PstI
FkoB-5CGGGATGGTTGTTGACGGC
FkoB-6CGTTTCACCGCTTTCATCGGG
Lac-DO-C CGCTACCATTACCAGTTGGTCTGG
LacZ-PE TAGCAGGCTCTTTCGATCC
Upf-N2attcagatgcatGGTAACCTTCAGACCGCTGTC NsiI
Upf-SattcaggcatgcTCACGGAAATGCCTTTCTGTGC SphI
Us1CCAGCGGTCGGTATGGAATGCG
Us2CGATGCGCGGACAGGGCggatccCGCTTAATG BamHI
  • a Capital letters indicate N. meningitidis-derived sequences, italicized capital letters indicate E. coli-derived sequences, lowercase letters indicate sequences added for cloning purposes, and underlined letters indicate recognition sites.

  • b Restriction enzyme sites added for cloning purposes.