TABLE 1.

Bacterial strains and plasmids

Strain or plasmidDescriptionReference or source
Strains
    NewmanWild type7
    ALC355Newman Δagr::tetM40
    ISP479CDerivative of 8325-436
    ISP479MuSpontaneous agrC mutant of ISP479CUnpublished data
    AS1ISP479Mu sae::Tn917This work
    AS2ISP479C sae::Tn917This work
    AS3Newman sae::Tn91717
    AS4Newman Δagr::tetM sae::Tn917This work
    RN6390Derivative of NCTC832531
    AS5RN6390 sae::Tn917This work
    DU5886Newman fnbA::TcrfnbB::EmrT. J. Foster Dublin
Plasmids
    pLT4tsTn917 delivery vector43
    pSN8388 cap5 promoter fused to the reporter gene xylE in pCL418
    pCWSAE7pGEMTeasy with 3.5-kb fragment encompassing ORF4, ORF3, saeS, and saeR of strain ISP479CThis work
    pCWSAE8pCRII with 3.5-kb fragment encompassing ORF4, ORF3, saeS, and saeR of strain NewmanThis work