TABLE 1.

Bacterial strains and plasmids

Strain or plasmidCharacteristicsReference or source
S. avermitilis 12804Prototrophic; pSA1 pSA2NCIMB, United Kingdom
S. avermitilis DM21912804 crgA::Tn1792This study
S. coelicolor M145Prototrophic; SCP1 SCP2 Pg1+12
S. coelicolor DC3845M145 ΔcrgA::tsrThis study
E. coli JM109F′ traD36 proA+B+ laclq Δ(lacZ)M15(lac-proAB) glnV44 e14 gyrA96 recA1 relA1 endA1 thi hsdR1730
E. coli ET12567(pUZ8002)dam13::Tn9 dcm-6 hsdM hsdR recF143 zjj-201::Tn10 galK2 galT22 ara14 lacY1 xyl-5 leuB6 thi-1 tonA31 rpsL136 hisG4 tsx-78 mtlI glnV44, containing the nontransmissible oriT mobilizing plasmid, pUZ800218, 21
SCH69Supercos containing chromosomal DNA from S. coelicolor; bla Km23
pOJ260Suicide vector for Streptomyces; oriT(RK2) aac(3)IV4
pOJ69pOJ260 containing ΔcrgA::tsrThis study
pSET152Integrative vector for Streptomyces; oriT(RK2) int attP (φC31) aac(3)IV4
pSET219pSET152 containing crgASaThis study
pSC3854pSET152 containing crgAScThis study
pIJ8600Integrative tipAp expression vector for Streptomyces; oriT(RK2) int attP (φC31) aac(3)IV28
pME41pIJ8600 containing tipAp crgA fusion cloning vector for E. coli; aac1 tsrThis study
pGB1P. Herron, unpublished data
pIJ2925Cloning vector for E. coli; bla15
pME38pIJ2925 containing crgAThis study
pME40pME38 containing crgA with 5′ NdeI siteThis study