TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant genotype and/or phenotypeReference(s) or source
E. coli DH5αF Δ(lacZYA-argF)U169 recA1 endA1 hsdR1 supE44 thi-1 gyrA96 relA17, 11
S. pneumoniae strains
    AC353TIGR4 Smr derivative8
    AC1213rlrA::magellan2 Smr Cmr9
    AC1272mgrA::magellan2 Smr CmrThis study
    AC1478malM-rlrA-cat-malP Smr Cmr9
    AC1481malM-mgrA-cat-malP Smr CmrThis study
    AC1500ΔmgrA Smr SpcrThis study
    STM206mgrA 5′-UTR::magellan2 Smr Cmr8
Plasmids
    pGEM-TCloning vector; AprPromega
    pCR-ScriptCloning vector; AprStratagene
    Amp SK(+)
    pEMCatContains magellan2; Apr/Cmr3
    PEMSpc/pR412Contains magellan5; Apr Spcr15
    pAC1000S. pneumoniae suicide vector9
    pCH84pAC1000 malm-rlrA-cat-malP; Smr Cmr9
    pAC1472pAC1000 malM-mgrA-cat-malP; Smr CmrThis study
    pAC1499pAC1000 ΔmgrA′::aad9′; Cmr SpcrThis study
    pAC1279pGEM-T rlrA RPA probe; Apr9
    pAC1280pGEM-T rrgA RPA probe; Apr9
    pAC1281pGEM-T rrgB RPA probe; Apr9
    pAC1285pGEM-T srtD RPA probe; Apr9
    pCH207pGEM-T mgrA RPA probe; AprThis study