TABLE 1.

Bacterial strains and plasmids

Strain or plasmidCharacteristicsSource or reference
Strains
    MC4100F araD139 Δ(argF-lac)U139 rpsL150 flbB5301 ptsF25 deoC1 thi-1 rbsR relA7
    RAM958MC4100 tolC::Tn10-4842
    RAM1149RAM958/pTrc99AThis study
    RAM1150RAM958/pTrc-tolC+ (BspHI-HindIII clone)This study
    RAM1151RAM958/pTrc-tolC(TolC1106N)This study
    RAM1152RAM958/pTrc-tolC(TolCS350F)This study
    RAM787MC4100 rcsB::Kmr22
    RAM789MC4100 rcsB::Kmr Δrfa-2057 (Cmr)22
    RAM913MC4100 ΦdegP::lacZ+ Tn10::zad-475R. Misra
    RAM914MC4100 ΦdegP::lacZ+ Δskp Tn10::zad-475R. Misra
    RAM951MC4100 surA::KmrR. Misra
    RAM1116RAM958 surA::Kmr/pTrc-tolC+This study
    RAM1117RAM958 Δrfa-2057/pTrc-tolC+This study
    XL1-RedendA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac mutD5 mutS mutT::Tn10Stratagene
Plasmid
    pTrc99AApr; expression vectorPharmacia