TABLE 1.

Transformation efficiencies of B. burgdorferi with pBSV2 or pKFSS1 in semisolid medium containing different antibioticsa

PlasmidNo. of colonies/μg of DNA (±SEM)
StreptomycinSpectinomycinKanamycin
pBSV2 (aphI)0 (±0)327 (±41)220 (±134)
pKFSS1 (aadA)79 (±27)695 (±136)0 (±0)
  • a Control transformations with no DNA yielded no colonies when plated on medium containing streptomycin or kanamycin, and the equivalent of 863 (±234) colonies when plated on medium containing spectinomycin.