TABLE 2.

Primers used

PrimerSequencea
LFhns-S5′-caaaataaGtcgaCttgcttaaaggc-3′ (SalI site)
RRhns-X5′-cgttattctAgacgaatatgagtccga-3′ (XbaI site)
Mar3-25′-ccggggacttatcagccaacctgttat-3′
S1B-CAT55′-ggtcgacggatccggggaat-3′
S1S-CAT35′-caagcaggtcgacatcttttctgttcat-3′
ompS1-PE5′-ttgctgcgcctgccactaataac-3′
ompA-PE5′-tttgcgcctcgttatcatccaa-3′
310b-15′-tagccttttatcatttattttatc-3′
310b-25′-atgagttatgtgttttgatttga-3′
310b-35′-caaagcatcaaatacatataaaaa-3′
310b-45′-tgtttctatttggttttttataatac-3′
310b-75′-attcttagtcacttatatatcctgtattat-3′
310b-85′-aatatgtagccacttcaacaaaac-3′
  • a Uppercase letter indicate changes in the primer sequence with respect to the wild type, designed to introduce restriction enzyme sites.