TABLE 2.

Oligonucleotides useda

UseNameSequence
PCRM219SCS5′ ACGTCTAGATGGTGCCTTTGGCGG TGT
M219SCA5′ CGGAATTCAAGCCTGTGGATAAC TCGGT
H69LHEcoRI5′ GCGGAATTCTTGTCCACCGTCAC ATCGTT
H69LHKpnI5′ CGCGGTACCTGATGCTGGTCGC CTGTCT
H69RHXbaI5′ GGCGTCTAGATCGTGGTGGGCT TCGGTTT
H69RHPstI5′ GGCCTGCAGCCGCACCTCGTCA CCAAGAT
SCH69S15′ ACGTCTAGATGACCAGGTAGTCG GGCT
SCH69A15′ CGGAATTCGCTGACCAGTGTTAT CGCA
Site-directed mutagenesiscrgA15′ CGACAGGAGAGACCCATATG CCGAAGTCACG
crgA25′ CGTGACTTCGGCATATGGGTC TCTCCTGTCG
  • a Restriction sites are in boldface.