TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype and/or descriptionaSource or reference
S. aureus strains
    ISP7948325 pig-13145
    RN42208325-4 r30
    MT5224c48325 nov (gyrB142) hisG15 pig 131 parC542 (Ser80Phe)46
    P188325 pig-131 flqA (parE), spontaneous premafloxacin resistanceThis study
    ISP21338325 pig-131 trp-489 Ω(chr::Tn917lac)246
    ISP21348325 pig-131 thrB484 Ω(chr::Tn917lac)146
    ISP225Ps5546
    DIP18-558325 pig-131, susceptible allelic exchange transformantThis study
    DIP18-68325 pig-131 flqA (parE), resistant allelic exchange transformantThis study
    DIP18-358325 pig-131 flqA (parE), resistant allelic exchange transformantThis study
E. coli DH5αFφ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 phoA hsdR17 (rK mK) supE44 λ thi-1 gyrA96 relA1GIBCO-BRL
Plasmids
    PGEM3-zf(+)3,199-bp cloning vector, AprPromega
    pCL52.18,119-bp plasmid containing the replicon of pGB2, Spr (E. coli), and the temperature-sensitive replicon of pE194, Tcr35
    pTrcHisA4.4-kb expression vector, AprInvitrogen
  • a Apr, ampicillin resistance; Spr, spectinomycin resistance; Tcr, tetracyclineresistance.